Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Biomed Chromatogr. 2015 May 26;29(11):1629–1636. doi: 10.1002/bmc.3470

Table 1.

CHROMATOGRAPHIC CONDITIONS

  1. Gradient Pump (Dionex GP50)

    1. Mobile Phase Composition: 88% Deionized Water & 12% 250 mM Sodium Hydroxide

    2. Flow Rate: 0.4 mL/minute

    3. High Pressure Limit: 2,000 psi

  2. Isocratic Pump for Rezex RCM Monosaccharide Columns (Bio-Rad 1350T)

    1. Mobile Phase Composition: 100% Deionized Water

    2. Flow Rate: 0.6 mL/minute

    3. High Pressure Limit: 256 kg/cm2

  3. Isocratic Pump for Dionex PA1 Column Cleanup (Bio-Rad 1350T)

    1. Mobile Phase Composition: 1.0 M Sodium Hydroxide

    2. Flow Rate: 0.4 mL/minute

    3. High Pressure Limit: 256 kg/cm2

  4. Column Heater

    1. Temperature: 65°C

  5. Autosampler (Dionex AS50)

    1. Injection Volume: 20 μL

    2. Run Time: 30 minutes

  6. Electrochemical Detector (Dionex ED50)

    1. Analog Range: 1 μC

    2. Waveform:

      1. 0.00 sec, E = +0.05 V

      2. 0.20 sec, E = +0.05 V

      3. 0.40 sec, E = +0.05 V

      4. 0.41 sec, E = +0.75 V

      5. 0.60 sec, E = +0.75 V

      6. 0.61 sec, E = −0.15 V

      7. 1.00 sec, E = −0.15 V

    3. Integration Period: 0.20 – 0.40 seconds

    4. gold electrode