Particle concentrations in formulations of buffer, OVA, buffer that contained emulsified silicone oil microdroplets, OVA that contained emulsified silicone oil microdroplets, OVA that contained syringe-extracted silicone oil microdroplets, and OVA formulations that contained alum. Particle concentrations were measured by FlowCAM® which detects particles between 2 - 100 μm in the formulations that were injected into mice on day 1 (solid) and day 15 (striped). Particle concentrations were three to five orders of magnitude higher for formulations that contained silicone oil microdroplets and alum microparticles compared to formulations of buffer or OVA. Error bars represent the standard deviation from the average of three replicates.