Skip to main content
. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: J Immunol Methods. 2015 Jun 20;425:69–78. doi: 10.1016/j.jim.2015.06.011

FIGURE 5. Proportions of immune subsets obtained by different enzymatic dissociation conditions.

FIGURE 5

The representation of the different immune cells as depicted by the mean percentages ± SD out of all live cells is plotted. The flow cytometry analysis was performed as previously described (Fig 4) and CD45 gating was utilized. n ≥3 side-by-side experiments using rhesus macaques. Lib-1hr-Ag: Liberase treatment for 1 hr with agitation, Lib-2hr-Ag: Liberase treatment for 2 hrs with agitation, Lib-2hr: Liberase treatment for 2 hrs without agitation, Lib-2hr-GM: Liberase treatment for 2 hrs without agitation followed by mechanical disruption with gentleMACS dissociator, M-GM: treatment using Miltenyi's enzyme mix for 2 × 30 min followed by 2 × mechanical disruption.