Table 1.

Enzymatic digestion alone or combined with mechanical disruption of tissues

Technical Steps	L-1hr-Ag*	L-2hr-Ag†	L-2hr‡	L-2hr C§	M¶
Muscle weight (g)	3	3	3	3	1
Epimysium	Yes	Yes	Yes	Yes	Yes
Enzymatic	Liberase	Liberase	Liberase	Liberase	Miltenyi Cocktail
Mechanical	No	No	No	C-tubes	C-tubes
Agitation	Yes	Yes	No	No	Yes
Incubation (min)	60	120	120	120	2 × 30
Enzyme quench	Yes	Yes	Yes	Yes	No
Filtration (70 μm)	Yes	Yes	Yes	Yes	Yes
Total time (hrs)	4:30	5:30	5:30	5:40	4:00

^*L-1hr-Ag: Liberase digestion of separated muscle and epimysium side-by-side for 1 hr, with agitation

^†L-2hr-Ag: Liberase digestion of separated muscle and epimysium side-by-side for 2 hrs, with agitation

^‡L-2hr : Liberase digestion of separated muscle and epimysium side-by-side for 2 hrs, no agitation

^§L-2hr C : Liberase digestion of separated muscle and epimysium as in L-2hr, followed by disruption of muscle in C-tubes

^¶M: Miltenyi enzyme digestion of separated muscle and epimysium combined in C-tubes followed by disruption in C-tubes