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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Transpl Immunol. 2015 Jul 21;33(2):125–129. doi: 10.1016/j.trim.2015.07.003

Figure 2. Skin allograft survival and mixed chimerism after donor bone marrow infusion with or without transient immunosuppression.

Figure 2

Figure 2

A: Survival of primary skin allografts. C57BL/6 mice were grafted with BALB/c skin at the same time they received different doses of donor bone marrow with or without immunosuppression. Filled circles: no bone marrow transplantation and no immunosuppression (negative control); filled squares: transient immunosuppression without bone marrow transplantation; open circles: transient immunosuppression with transfer of 20 million bone marrow cells; slashed circles: transient immunosuppression with transfer of 20 million bone marrow cells one hour after AMD3100 injection; filled diamonds: transient immunosuppression with transfer of 50 million bone marrow cells; open squares: transient immunosuppression with transfer of 100 million bone marrow cells. B: Survival of secondary skin allografts. Recipient C57BL/6 mice of BALB/c primary skin allografts were re-grafted with BALB/c donor-type and CBA/Ca third-party skin allografts from 120 days after primary skin grafting. Dashed lines: CBA/Ca allografts. Solid lines: BALB/c allografts. Filled circles: no bone marrow transplantation and no immunosuppression (negative control); filled squares: transient immunosuppression without bone marrow transplantation; open circles: transient immunosuppression with transfer of 20 million bone marrow cells; slashed circles: transient immunosuppression with transfer of 20 million bone marrow cells one hour after AMD3100 injection; filled diamonds: transient immunosuppression with transfer of 50 million bone marrow cells; open squares: transient immunosuppression with transfer of 100 million bone marrow cells. C: Mixed hematopoietic chimerism and circulating T lymphocyte levels in peripheral blood 90 days after mice received 0, 20, 50 and 100 million donor bone marrow cells with or without transient immunosuppression.