Table 1.
ANOVA for various eco-physiological traits measured in genotype 592 and 520 grown in a growth chamber (three blocks) and exposed to two experimental conditions: well-watered or drought
| Source of variation | ||||||
|---|---|---|---|---|---|---|
| Traits | Block | Genotype (G) | Treatment (T) | G x T | Error | |
| (d.f. = 2) | (d.f. = 1) | (d.f. = 1) | (d.f. = 1) | (d.f. = 52) | ||
| Total plant DWa (g) | MS | 0.048 | 0.055 | 20.355 | 0.315 | 0.059 |
| p-value | 0.452 | 0.338 | <0.001 | 0.025 | ||
| Total leaf N pool (g) | MS | 0.020 | 0.024 | 0.972 | 0.034 | 0.005 |
| p-value | 0.030 | 0.038 | <0.001 | 0.014 | ||
| RGRa (g g−1 wk−1) | MS | 0.003 | 0.006 | 1.188 | 0.018 | 0.003 |
| p-value | 0.456 | 0.184 | <0.001 | 0.025 | ||
| Number of syll. shoots | MS | 50 | 2269 | 1050 | 874 | 46 |
| p-value | 0.342 | <0.001 | <0.001 | <0.001 | ||
| Leaf DWa (g) | MS | 0.343 | 0.857 | 31.523 | 0.057 | 0.302 |
| p-value | 0.328 | 0.098 | <0.001 | 0.665 | ||
| LW/W (%) | MS | 108 | 2640 | 399 | 1465 | 220 |
| p-value | 0.614 | <0.001 | 0.183 | 0.013 | ||
| LARa (m2 g−1) | MS | 2.87 | 97.30 | 13.57 | 1.39 | 0.92 |
| p-value | 0.052 | <0.001 | <0.001 | 0.223 | ||
| SLAa (m2 kg−1) | MS | 58.0 | 42.2 | 390.8 | 10.7 | 12.7 |
| p-value | 0.015 | 0.074 | <0.001 | 0.363 | ||
| Shoot DWa (g) | MS | 0.018 | 0.077 | 22.310 | 0.042 | 0.067 |
| p-value | 0.768 | 0.289 | <0.001 | 0.434 | ||
| SW/W (%) | MS | 441 | 1410 | 15 | 2190 | 223 |
| p-value | 0.149 | 0.015 | 0.787 | 0.003 | ||
| LAPa (g m−2 wk−1) | MS | 68 | 728 | 8080 | 5 | 40 |
| p-value | 0.191 | <0.001 | <0.001 | 0.729 | ||
| SPADa (rel. units) | MS | 3.6 | 844.1 | 46.9 | 2.3 | 8.1 |
| p-value | 0.645 | <0.001 | 0.020 | 0.596 | ||
| Root DWa (g) | MS | 0.476 | 0.321 | 15.384 | 0.405 | 0.126 |
| p-value | 0.029 | 0.116 | ≤0.001 | 0.079 | ||
| RW/W (%) | MS | 2490 | 31 | 4997 | 643 | 114 |
| p-value | <0.001 | 0.606 | <0.001 | 0.021 | ||
| No. of leaves | MS | 546 | 2640 | 36803 | 1995 | 231 |
| p-value | 0.103 | 0.001 | <0.001 | 0.005 | ||
| Leaf temperature (°C) | MS | 9.56 | 0.19 | 13.56 | 1.26 | 0.70 |
| p-value | <0.001 | 0.607 | <0.001 | 0.185 | ||
| RWCa (%) | MS | 105.1 | 86.7 | 818.2 | 132.0 | 199.3 |
| p-value | 0.595 | 0.513 | 0.048 | 0.420 | ||
| Leaf Δ13C (‰) | MS | 1.7 | 40.6 | 138.8 | 14.5 | 1.1 |
| p-value | 0.224 | <0.001 | <0.001 | 0.001 | ||
| Leaf Δ18O (‰) | MS | 0.16 | 0.12 | 59.12 | 5.94 | 0.27 |
| p-value | 0.555 | 0.503 | <0.001 | <0.001 | ||
| Leaf Δ13C/Δ18O | MS | 0.002 | 0.035 | 0.242 | 0.023 | 0.001 |
| p-value | 0.190 | <0.001 | <0.001 | <0.001 | ||
The ANOVA model included the main effects Block, Genotype and Treatment and the Genotype x Treatment interaction; Block was treated as random factor, and Genotype and Treatment were treated as fixed factors. MS = mean square. d.f. = degrees of freedom
aTrait abbreviations: DW dry weight (biomass), RGR relative growth rate, LAR leaf area ratio, LAP leaf area productivity, SLA specific leaf area, SPAD values indicate leaf chlorophyll content (here assessed two days before the final harvest), RWC relative water content P-values ≤ 0.05 are shown in bold