Table 2.
Characteristics of the DNA barcodes evaluated in this study
| DNA region | ITS2 | psbA-trnH | ITS2 + psbA-trnH |
|---|---|---|---|
| Number of individuals | 86 | 75 | 75 |
| Number of species | 7 | 7 | 7 |
| PCR/sequencing success (%) | 100/100 | 100/100 | 100/100 |
| Amplified sequence length (bp) | 221–223 | 300–313 | 521–530 |
| Aligned sequence length (bp) | 227 | 320 | 547 |
| Average GC content (%) | 52.72 | 25.62 | 37.18 |
| Variable sites | 43 | 19 | 59 |
| Haplotypes | 23 | 23 | 28 |
| Intra-specific distance range (mean) | 0–0.0571 (0.0041) | 0–0.0340 (0.0021) | 0–0.0297 (0.0025) |
| Inter-specific distance range (mean) | 0–0.1298 (0.0594) | 0–0.0489 (0.0237) | 0.0019–0.0708 (0.0363) |