Table 1.
Otolith formation in MO or mRNA of OlPKS
| Experiment | Nucleltide | Fish | 4 otoliths | 1-3 otoliths | No otolith | n |
|---|---|---|---|---|---|---|
| Phenocopy | olpks first-Met MO | wt | 0% | 0% | 100% | 39 |
| Rescue | (uninjected) | ha | 0% | 0% | 100% | 31 |
| olpks mRNA | 73% | 23% | 3% | 30 | ||
| Active site mutation | (uninjected) | ki79 | 0% | 0% | 100% | 14 |
| olpks-mRNA | 65% | 35% | 0% | 43 | ||
| KS*-mRNA | 0% | 0% | 100% | 15 | ||
| AT*-mRNA | 0% | 2% | 98% | 57 | ||
| DH*-mRNA | 0% | 0% | 100% | 18 | ||
| KR*-mRNA | 0% | 0% | 100% | 17 | ||
| ACP*-mRNA | 0% | 0% | 100% | 27 | ||
| Loop*-mRNA | 53% | 40% | 7% | 60 |
Asterisks indicate one amino acid mutation are introduced.
‘Loop’: interdomain region.
Fully rescued: 4 otoliths in one animal.
Partially rescued: 1–3 otoliths in one animal.
Not rescued: no otolith.