Figure 4.

A multiple alignment of partial nucleotide sequences of the 6th exon of GNAT3 . Human, mouse and cow GNAT3 sequences were shown in addition to that of cetaceans and artiodactyls sequenced (shown with red fonts) or annotated in this study. Numbers above the alignment are corresponding to the nucleotide positions in the human GNAT3. Sequences based on Sanger-sequencing technology are indicated by red fonts. Asterisks under the alignment indicate non-variable nucleotide positions. Disrupting mutations and their positions are shaded. ‘Hap1’ and ‘hap2’ indicate haplotypes. All cetaceans share two lineage-specific pseudogenization mutations (a 1-bp deletion in the position 643 and a premature stop codon in the position 658–660).