Fig 2. Anti-proliferative activity of Bp4eT (A) and its metabolites, Bp4eA (B), and both E (C) and Z (D) isomers of Bp4eS.

For determination of anti-proliferative activity, the cancer cell lines (i.e., HL-60, MCF-7, HCT116 and A549) and non-cancer cell lines (i.e., H9c2 and 3T3) were incubated with the agents for 72 h/37°C and proliferation then assessed using the MTT assay. The results are mean ± SD (n ≥ 4 experiments).