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. 2015 Oct 13;10(10):e0139957. doi: 10.1371/journal.pone.0139957

Fig 1. The GFP-ML1Nx2 probe labels endosomal compartments.

Fig 1

(A) GFP-ML1Nx2 co-localizes more with PtdIns3P than other constitutive inositol lipids; the “co-localization” images show the images of the normalized mean deviation product (nMDP) between two channels, wherein bright pixels that correlate in each channel appear gold and pixels that anti-correlate appear green (dim pixels that correlate in each channel are shaded black for clarity). The graph at right shows the mean nMDP score ± 95% C.I. for 30 cells, with the P values represented above (One-way ANOVA with Tukey’s multiple comparison; *** = P < 0.0001). (B) GFP-ML1Nx2 co-localizes to a similar extent with early (Rab5), and late endo/lysosomal (Rab7 and LAMP1) markers. “Co-localization” images as in A; graph at right shows the mean nMDP score ± 95% C.I. for 30 cells, with the P values represented above (One-way ANOVA, no significant variance between groups). Scale bar = 15 μm and applies to A and B.