Fig. 4.

Effect of MLE on plasma and hepatic oxidative stress-related markers in high-fat diet-induced obese mice. a Protein levels of 4-HNE, b HO-1 activity, c GPx activity, and d protein levels of nuclear Nrf2 in hepatic tissues. Protein samples were pooled from 6 mice per group. A representative image of repeated experiments is shown in the left panel. The bands show the intensity of the bands that were densitometrically measured and normalized to the band levels of β-actin. Values are mean ± SEM and tested by ANOVA with Duncan’s test for multiple comparisons. Values with the same superscript letter are not significantly different (P < 0.05). CON, normal control diet-fed mice; HF, high-fat diet-fed control; L, low-dose mulberry leaf extract (133 mg/kg)-treated high-fat diet-fed mice; H, high-dose mulberry leaf extract (666 mg/kg)-treated high-fat diet-fed mice