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(A) Fluorescence microscopy of four grains expressing GFP driven by the oat GLO1 promoter. (B) Quantification of GFP based on Western blotting with an anti GFP antibody. (C) Total soluble protein profile obtained by Coomassie Brilliant Blue staining of 12% SDS-PAGE separations of extracts of a T3 selection of line BG136/1E02-14-4 expressing GFP.
