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. 2015 Oct 13;10(10):e0140476. doi: 10.1371/journal.pone.0140476

Fig 2. Transverse grain sections demonstrating the site of GFP accumulation (green) and cell wall autofluorescence (yellow).

Fig 2

(A) GFP accumulation throughout the aleurone and endosperm of grains set by plants harboring the AsGLO1P::GFP transgene. (B) In a transgenic barley line constitutively expressing GFP (ZmUBI1P::GFP), GFP fluorescence was restricted to the aleurone layer. (C) In a wild type grain, no GFP signal was detectable. (D) Higher magnification of an endosperm cell reveals discrete deposits of GFP within the cytoplasm of the transgenic line shown in (a). (E) The cytoplasm of a wild type endosperm cell lacks any GFP signal. Bar: 50 μm in (A-C), 10 μm in (D-E).