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. 2015 Oct 13;10(10):e0140476. doi: 10.1371/journal.pone.0140476

Fig 3. Detection of 2G12 antibodies in grain extracts.

Fig 3

Grain extracts and CHO cell-derived 2G12 standards were separated by gradient SDS-PAGE under non-reducing conditions. 2G12 antibodies were detected by their binding to anti-human IgG Fcg-peroxidase. Lanes 1–3: 0.5, 1.0 and 2.0 μg of protein extracted from BG208/1E06-P4. Lanes 4–6: 0.5, 1.0 and 2.0 μg of protein extracted from BG208/1E06-P7. Lanes 7–9: 0.5, 1.0 and 2.0 μg of protein extracted from BG208/1E06-P13. Lanes 10–13: 0.5, 1.0, 2.0 and 4.0 ng 2G12 obtained from CHO cells.