Table 2. Comparison of different stable transgenic expression systems for the production of 2G12 mAB in plants.
Species | Tag | Organ | Promoter | Yield | Reference | |
---|---|---|---|---|---|---|
Tobacco | - | Leaf | CaMV 35S | 17–18 μg/mL | (45 μg/g)* | [37] |
KDEL KDELELP, KDEL | leafseedLeaf | CaMV 35S Faba bean USPCaMV 35S | 0.4% of TSP0,3% of TSP1% of TSP | (2.8 μg/g)**(1.7 μg/g)**(6.9 μg/g)** | [11][11][11] | |
ELP, KDEL | Seed | Faba bean USP | 1% of TSP | (5.8 μg/g)** | [11] | |
Maize | KDEL- | Kernels/ endospermKernels/ endosperm | Rice Glut1Rice Glut1 | 30 μg/g75 μg/g | [5][6] | |
Rice | - | Kernels/ endosperm | Rice Glut1 | 46 μg/g | [33] | |
Barley | KDEL | Grain/ endosperm | Oat Glo1 | 0.4% of TSP | (160 μg/g) | this study |
* line selected over several generations and optimized conditions for growth and harvest
**units were converted based on the average TSP values obtained with the respective extraction method [10]