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Primer and probe sequences used in quantitative real-time polymerase chain reaction
| Targeted gene | Sequence (5’ to 3’) | Purpose | Product size (base pairs) |
|---|---|---|---|
| Tax | ATCCC GTGG AGACTCCTCAA | Forward | 100 |
| CCTGGGAAGTGGGCCATG | Reverse | ||
| CATGCCCAAGACCCGTCGGAGG | Probe | ||
| HBZ | CTCGACCTGAGCTTTAAACTTACC | Forward | 133 |
| CATGACACAG GCAAGCATCG | Reverse | ||
| CGGACGCAGTTCAGGAGGCACCAC | Probe |
