Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Oct 13;10(10):e0140143. doi: 10.1371/journal.pone.0140143

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Forni et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 4 — The mesenchymal stem cells from murine dermis were selected for their ability to form colonies at low densities (CFU), as shown in the micrograph (A). These colonies expand and form a confluent layer at P0, as shown in (A). These cells are then passaged whenever they reached 80% confluence and expanded until passage 10 (B). Cells at P4 were used to detect the presence of positive and negative markers of MSCs by flow cytometry, namely: CD34, CD31, CD45, CD44, CD90, CD29 and CD105 (C). All groups marked with conjugated antibody (blue line) were compared to their respective controls (gray filled line); at least 50.000 events were collected for analysis. IgG isotype controls conjugated with Alexa 488 and APC were used as negative controls. Results are representative of those obtained in three independent experiments performed in triplicates.