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. 2015 Oct 14;35(41):13975–13988. doi: 10.1523/JNEUROSCI.1937-15.2015

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Copyright © 2015 the authors 0270-6474/15/3513976-14$15.00/0

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Figure 7. — Expression and functionality of the adolescent CB1R and behavioral characterization of adolescent and adult Fischer 344 rats. Striatal CB1R protein levels were higher in adolescent compared with adult rats (p = 0.036) (a). No differences were found for PPR (age effect: F_(1,83) = 3.2, p = 0.08) Representative traces for PPR: EPSC amplitudes are normalized to the peak of the first EPSC. In each example, there are three trials superposed (for three different interstimulus intervals). Scale bar, 20 ms. (b). The CB1R antagonist/inverse agonist SR141716 (SR) was found to exert a stronger effect in adolescent compared with adult rats on fEPSPs (p = 0.033). Representative traces of fEPSPs recorded before (black) and after (gray) application of SR141716. Scale bar, 0.1 mV; 2 ms (c). The amplitude of sEPSCs did not differ between the two groups (p = 0.08), but, as in MT rats, the sEPSC interevent interval was significantly enhanced in adolescent rats (p = 0.012). Representative traces for sEPSC: There are 7 consecutive seconds for each condition (two rows per each). Scale bar, 10 pA; 500 ms (d). Adolescent animals were further screened for a selection of behavioral characteristics observed before in MT animals. Adolescent rats showed increased risk-taking in the EPM (e) compared with adult Fischer rats (EPM: percentage time: p = 0.009; percentage entries: p = 0.028). Exploration of a novel object (f) was also increased (p = 0.009). Adolescent Fischer rats showed an increase in reward-related behaviors compared with adult rats. Intake of a palatable food reward (g), as well as PR responding (h), was significantly higher in adolescents than adults (intake: p = 0.001; PR testing: p = 0.004). Social play behavior was also strongly increased in adolescent animals (i) compared with adult controls (attacks received, initiated and pinning: p < 0.001). Finally, the acute stimulatory effects of cocaine (j) were significantly enhanced in adolescence compared with adulthood (F_(2,40) = 3.3, p = 0.047). Data are indicated as means ± SEM (CB1R Western blot: n = 5; PPR: adolescent: n = 11, adult n = 12; sEPSC: adolescent: n = 11, adult n = 10; effects of SR on fEPSP: n = 6; EPM, novel object exploration, food reward intake, PR: n = 12; social play: n = 9; cocaine dose–response curve: adolescent: n = 12, adult n = 10).