Figure 7.

The neonatal loss of oligodendrocytes correlates with a truncated OPC proliferative response to adult demyelination. A, B, LPC injected into WT animals resulted in a surge of OPC proliferation (Olig2⁺Ki67⁺) from 5 to 14 dpl. C, D, By contrast, a proliferative surge in MBP-iCP9 animals was conspicuously absent during this postlesion time frame despite an equivalent initial proliferative response from 0 to 5 dpl. E, However, at 5 dpl, there was a significant reduction in dorsal column Olig2⁺ cells in MBP-iCP9 versus WT that could not be explained by a decrease in the number of Ki67⁺ proliferating cells. F, By 14 dpl, however, there was a decrease in Olig2⁺ cells, Ki67⁺ cells, and double-labeled cells, suggesting an unsustained recruitment of proliferating OPCs from 5 to 14 dpl in MBP-iCP9 animals compared with WT littermate controls. n = 3 control. n = 5 experimental. Scale bar, 25 μm. *p < 0.05, **p < 0.01. Error bars indicate SEM.