Table 2.

Features of miniDNA vectors compared to standard plasmid DNA vectors.

General features	Plasmid DNA	MiniDNA*
	Replicative in bacterial host	Non-replicative in bacterial host
	Supercoiled	Supercoiled or circular/linear closed
	Bacterial origin of replication	No bacterial origin of replication
	Bacterial selection marker (Antibiotic resistance gene)	No bacterial selection marker (Antibiotic resistance gene)
	Relatively high immunostimulatory unmethylated CpG dinucleotides**	No to very few immunostimulatory unmethylated CpG dinucleotides
	Large-scaled production is available	Large-scaled production is available
	Pharmaceutical-grade purification is available	Pharmaceutical-grade purification is available
	Medium to large size***	Relatively smaller than plasmids

^*Bacterial sequence depleted.

^**Depends to the size.

^***Depends to the size of therapeutic transgene.