FIGURE 5.

YKL40 was detected with Alexa Fluor 594-conjugated Affini Pure Goat-anti-Rabbit IgG (Red). Most of the cells in immunostaining for YKL40 show a characteristic distribution pattern, the signal being localized in the cytoplasm and in the nucleo in cell treated with BO. The Blue represents DAPI nuclear counterstain. It is important to note the many nuclei present per cell. Scale bars equal 10 μm. (A,E,I) Double staining of YKL40 in red and nuclei in blue. (B,F,J) CHIT1 staining in red alone. (C,G,K) nuclear staining with DAPI alone. (D,H,L) Z-stack confocal images, Double staining of YKL40 in red and nuclei in blue.