Table 3B.

Primers and estimated PCR products for 12 pairs of selected PHT unigenes.

Numbers	Unigene pair (5′ end + 3′ end)	Reference location	Possible gap	Primer: forward/reverse (5′–3′)	Designed PCR size (bp)	Estimated size (bp)
1	Unigene91380_All + Unigene105912_All	82–170+348–448	178 aa	TAGTTGTGGAGGAGAAATGG/AGGATGGAGAAGGTGACG	558	1092
2	Unigene5546_All + Unigene11489_All	5′–157+229–319	72 aa	CTTTTGGGGCGTCTGTA/GAACCACGTGCTTGTGG	553	769
3	Unigene5546_All + Unigene47851_All	5′–157+166–3′	9 aa	CTTTTGGGGCGTCTGTAC/TTCCCTTCGCTTTTTGTG	677	704
4	Unigene16022_All + Unigene50223_All	5′–91+417–3′	326 aa	ATTTCATCACACACCCAG/ATTTACCCATAGACTCCG	854	1832
5	Unigene16022_All + Unigene90558_All	5′–91+417–3′	326 aa	ATTTCATCACACACCCAGA/CAAGAGATTTACCCATAGACTC	860	1838
6	Unigene11489_All + Unigene63539_All	229–319+412–493	93 aa	CAGATAGACGCCGATGAGG/GCAACCAAGAACAATAAGAGAG	416	695
7	Unigene48391_All +Unigene44133_All	5′–63+404–3′	341 aa	CTTTCCACCTCAAGTCAT/GTGCAACCAAGAACAATAAGA	587	1610
8	Unigene53055_All + Unigene44133_All	5′–163+404–3′	241 aa	TCTGTATCTCCCTCCTAAC/CCAAGAACAATAAGAGAGTT	632	1355
9	Unigene125621_All + Unigene61379_All	5′–68+131–277	63 aa	CCCCATCTCCACTCCTGTT/AAGGTTGACGGTGGTGTTC	566	755
10	Unigene141416_All + Unigene129694_All	5′–233+210–281	none	CTGCTATCACCCCTCTTG/CCTTGGTTCAATTTTTATCC	745	745
11	Unigene141415_All + Unigene129694_All	8–87+210–281	123 aa	AAAGTATAATGGTAGGAAGTCC/GTTCAATTTTTATCCTCAGG	458	827
12	Unigene29837_All + Unigene46396_All	5′–230+292–3′	62 aa	GTGTTGCTTTGTGGTCCT/CCCATGTAATATTCCGGT	962	1148

Reference location indicated the location of unigene coding protein aligning with protein database of Arabidopsis thaliana. aa, amino acid; bp, base pair.