Figure 2.

Expression of pluripotency markers are upregulated in BM-derived L-MPPs post-irradiation with ¹H or ⁵⁶Fe particles. After whole-body irradiation with 0.5 Gy, 1 GeV ¹H and 0.15 Gy, 1 GeV/n ⁵⁶Fe particles, mononuclear cells from bone marrow of C57BL/6NT mice were sorted into L-MPPs (CD34⁺/c-kit⁺/Sca-1⁺/AC133⁻/Lin⁻) by FACS at multiple time points over 40 weeks post-IR. Levels of (A) Nanog, (B) Oct4, and (C) Sox2 were analyzed using Taqman probes by qRT-PCR. Relative mRNA levels were calculated with respect to control sham irradiated animals. Bars represent mean ± SEM (n = 6/group) for control (solid black bars), ¹H-IR (solid blue bars), and ⁵⁶Fe-IR (solid red bars). “*” represents statistically significant differences compared to control with p < 0.05.