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. 2015 Oct 1;29(19):2054–2066. doi: 10.1101/gad.267245.115

Figure 2.

Figure 2.

Alternative splicing of MKK7 positively regulates JNK signaling. (A) Schematic of the MKK7 5′ splice site-blocking AMO on the pre-mRNA and its effect on the MKK7 protein. (B) Representative RT–PCR gel of MKK7-E2 and average percent inclusion (n > 3) for Jurkat T cells transfected with a control AMO against a splice site in an unrelated gene (Cont) or the AMO blocking the 5′ splice site of MKK7-E2 (MKK7) in the presence and absence of PMA (48 h). (C) Corresponding Western blot of Jurkat T cells treated as in B, blotted for phospho-c-Jun (Ser73), total c-Jun ,and total MKK7 (PMA, 2 h). (D) Quantification of TNF-α mRNA normalized to B2M by RT–PCR (n = 4) in cells treated as in B (PMA, 48 h). Error bars represent standard deviation. (*) P < 0.005, Student's t-test.