Figure 7.

JNK signaling and CELF2 coregulate alternative splicing in T cells. (A) Pie chart of the overlap of significant JNK-dependent alternative splicing events from Figure 6 with published CELF2-dependent alternative splicing events (Mallory et al. 2015) and a list of genes containing alternative exons that are coregulated by JNK and CELF2. (B) Enriched motifs around JNK-responsive exons in primary CD4⁺ T cells as determined by the AVISPA algorithm. (C) Analysis of two cassette exons in two JNK-dependent alternative splicing events that are predicted to be coregulated by CELF2 on the basis of containing an AVISPA-determined CELF2 motif. Representative RT–PCR gel quantifying the percent inclusion in wild-type (WT) versus CELF2 knockdown (KD) samples from Jurkat T cells.