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. 2015 Oct 13;59(11):6741–6748. doi: 10.1128/AAC.00618-15

FIG 3.

FIG 3

Steady-state expression levels of wild-type (WT) and mutant CTX-M-14 β-lactamases. (A) Immunoblot analysis showing protein expression levels in the periplasmic fraction of E. coli cultures carrying blaCTX-M-14 with the A77V, D240G, P167S, A77V/D240G, and A77V/P167S mutations on the CTX-M-14-pTP123 plasmid. (B) Immunoblot analysis showing protein expression levels of the A76E and A77V/A76E CTX-M β-lactamases. The density of the bands was quantified using ImageJ 64-bit software and plotted relative to that of the WT enzyme.