TABLE 2.

Primers used for site-directed mutagenesis to create the mutant CTX-M β-lactamases in this study

Primer	Sequence
A76E-F	5′-AGTAAAGTTATGGAGGCCGCGGCG-3′
A77V-F	5′-CAGTAAAGTTATGGCGAACGCGGCGGTGCTTAAG-3′
A77V-R	5′-CTTAAGCACCGCCGCGTTCGCCATAACTTTACTG-3′
D240G-F	5′-CCGGCAGCGGCGGCTACGGCACCAC-3′
D240G-R	5′-GTGGTGCCGTAGCCGCCGCTGCCGG-3′
P167S-F	5′- CTGGATCGCACTGAAAGCACGCTGAATACCGCC-3′
P167S-R	5′-GGCGGTATTCAGCGTGCTTTCAGTGCGATCCAG-3′