FIG 2.

Membrane integrity assays by measuring propidium iodide (PI) uptake. The addition of sublancin at the indicated concentrations (black bars) does not alter the membrane permeability of B. halodurans C-125 (a) and B. subtilis ATCC 6633 (b). Nisin was used as the positive control (gray bars). *, P < 0.05 between nisin (0.1 μM to 20 μM)-treated cells relative to no drug. In all experiments in which the cells were exposed to sublancin, the increase in MFI relative to the control was not statistically significant (P > 0.05). The means of the data from one experiment conducted in triplicate are shown. The data are representative of the results from three independent experiments. The error bars indicate standard deviations.