FIG 2.

Rv2887 is in the MarR family. (A) Alignment of Rv2887 to the consensus sequence for Pfam01047 (MarR) and four other MarR family proteins (selected as the five most diverse members in the conserved domain database, including the consensus sequence) (14). The bottom row is the consistency, as estimated by PRALINE (17). (B) Secondary structure prediction from Phyre2 for Rv2887 (16). (C) Secondary structure prediction for the C terminus of mutant 3. The black boxes in panels A and B indicate V61, which is mutated to alanine in mutant 2.