TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype/description | Source |
|---|---|---|
| Brucella melitensis | ||
| B. melitensis 16M | Wild-type strain | Laboratory collection |
| CMR27 | ΔbveA::Kmr | This work |
| CMR28 | CMR27 complemented with pCR108 carrying bveA | This work |
| TOK09 | CMR27 carrying a chromosomal insertion of pTK19 in the tRNAtrp-secE intergenic region | This work |
| E. coli | ||
| Origami 2(DE3)pLysS | Host for expression of BveA fusion proteins | Novagen |
| Salmonella enterica serotype Typhimurium | ||
| IR715 | Spontaneous nalidixic acid-resistant derivative of ATCC 14028 | Laboratory collection |
| Plasmids | ||
| pBluescript KS | ColE1, Apr | Stratagene |
| pCR2.1-TOPO | TA cloning vector | Invitrogen |
| pUC4-KSAC | Source of Tn903 kanamycin resistance cassette | Pharmacia |
| pCR105 | Construct for allelic exchange to delete B. melitensis bveA, Kmr | This work |
| pCR108 | Carries the bveA gene in pBBR1MCS4 vector background, Apr | This work |
| pTK19 | Carries the bveA gene from B. melitensis 16M in exchange for the mCherry gene of pKSoriT-bla-PsecE-mCherry (13), Apr | This work |
| pTK07 | Overexpression construct of PelB-BveA-6×His fusion protein in E. coli; pET25b(+), Apr; pLac | This work |
| pTK25 | Overexpression construct of MBP-BveA fusion protein in E. coli; pMALp5x, Apr; pLac | This work |