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. 2015 Nov;21(11):1859–1865. doi: 10.1261/rna.051292.115

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© 2015 Abeysirigunawardena and Woodson; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 1. — Assembly of the 16S 5′ domain. (A) Proteins S4 (pink), S17 (green), S16 (blue), and S20 (wheat) bound to nucleotides 21–556 of the Escherichia coli 16S rRNA 5′-domain RNA (gray) in the 70S ribosome (2i2p). Green and red spheres indicate positions of Cy3 and Cy5, respectively. (B) Thermodynamic cycles for assembly of the 16S 5′ domain. Thermodynamically favorable steps predicted by the Nomura map are shown with thick arrows. Green and red arrows show a preference for the native and intermediate S4-5′-domain RNA complexes, respectively.