FIGURE 2.

Characterization of M. lignano piRNAs. (A) Size distribution of putative piRNA population in M. lignano. Numbers of uncollapsed and collapsed reads per million total mapping reads (RPM) aligned to the genome are plotted on the left and right, respectively. (B) Sequence bias of the 28–32-nt long RNA population from whole worms. (C) Distribution of 5′ overlap between sense and antisense piRNA strands. Z-score represents the enrichment of 10-bp overlap. (D) Examples of unidirectional and bidirectional piRNA clusters from the genome draft. The piRNA coverage at each nucleotide position of the clusters is shown in gray.