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. 2015 Nov;21(11):1885–1897. doi: 10.1261/rna.052456.115

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© 2015 Zhou et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 3. — Expression patterns of Macpiwi1 and Macpiwi2 in M. lignano. (A) Interference contract image and diagrammatic representation of an adult worm. (B) Immunofluorescence labeling showing localization of Macpiwi1 (green) in adult worms. Dividing cells are labeled with EdU (red). (h) Head, (t) testis, (o) ovary, (de) developing egg, (me) mature egg. Stars denote eyes. Arrow points to nonspecific staining by the secondary antibody. (C) Localization of Macpiwi2 mRNA by whole-mount in situ hybridization in adult worms, hatchlings, and during regeneration induced by posterior amputation. Sense riboprobe was used as negative control. Arrows point to blastemas during regeneration. (t) Testis, (o) ovary, (de) developing egg, (me) mature egg.