Figure 2. Phylogenetic tree of E2 families with the consensus patterns for the positions corresponding to the CES/D site.

In the panel on the right, we reported a portion of the sequence alignment reported in Supplementary Fig. S1 to show the residues in the immediate proximity of the CES/D site. Each sequence is labelled as ‘Organism_E2_Family’ so for example S. cerevisiae Ubc2 (Rad6) from family 2 is labelled as ‘Sc_Ubc2_2’. The phylogenetic tree is coloured according to the conserved motif at the CES/D site within each family using light blue, light green, marine, yellow, pink and violet colours for DP, SP, SP/DP, SP/SA, EP/EA and E sequence motifs, respectively. The NCUBE families are highlighted in orange.