Table 1. Vectors and Y2H PPI summary.

Bait/prey vectorcombination	Tag fusions	Intraviralinteractionsa	ORF2 interdomaininteractionsa
pGBKT7g/pGADT7g	NN (N-terminal fusions)	31	1
pGBKT7g/pGADCg	NC (N-terminal/C-terminal fusions)	23	16
pGBKCg/pGADT7g	CN (C-terminal/N-terminal fusions)	21	19
pGBKCg/pGADCg	CC (C-terminal fusions)	10	10

Tag fusions indicate the location of the DNA-binding (DBD) and transactivation domain (AD) of the resulting hybrid proteins in the corresponding vector combination.

^aRedundant interaction sets. Included are interactions found with different vector combinations.