Table 2. Intraviral protein interactions from Y2H screening.
| InteractionNo. | Bait | Prey | NN | NC | CN | CC | N°Vectorpairs | Redundant PPI inY2H (PPI No.) | Scoredon X mM3-AT | LuMPIS | PPIQuality |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Met | X | + | + | + | + | 4 | r, 18 | 0 | + | high |
| 2 | Met | O3 | + | + | + | + | 4 | 0 | + | high | |
| 3 | Met | Plp | + | − | + | − | 2 | 4, 12 | 0 | − | medium |
| 4 | Met | Pvx | + | − | + | − | 2 | r, 3, 12 | 0 | + | high |
| 5 | Y | X | + | − | − | − | 1 | 0 | + | medium | |
| 6 | Plp | Plp | + | + | + | + | 4 | s, 8, 10, 11 | 0 | + | high |
| 7 | Plp | X | + | + | − | − | 2 | r, 13, 19, 21 | 0 | n/a | medium |
| 8 | Plp | Pvx | + | + | + | − | 3 | r, 6, 10, 11 | 0 | + | high |
| 9 | Plp | O3 | + | + | − | − | 2 | r, 14, 31, 33 | 0 | + | high |
| 10 | Pvx | Plp | + | + | + | − | 3 | r, 6, 8, 11 | 3 | + | high |
| 11 | Pvx | Pvx | + | + | + | + | 4 | s, 6, 8, 10, 15, 17 | 3 | + | high |
| 12 | Pvx | Met | + | + | − | − | 2 | r, 3, 4 | 3 | + | high |
| 13 | Pvx | X | + | + | − | − | 2 | r, 7, 19, 20, 21 | 3 | + | high |
| 14 | Pvx | O3 | + | + | − | − | 2 | r, 9, 23, 31, 32, 33 | 3 | + | high |
| 15 | Pvx | V | − | − | + | − | 1 | 11, 17 | 3 | − | medium |
| 16 | Pvx | Rdrp | + | − | − | − | 1 | r, 26, 27, 28 | 3 | + | high |
| 17 | V | V | − | + | + | − | 2 | s, 15, 11 | 3 | − | medium |
| 18 | X | Met | + | + | + | + | 4 | r, 1 | 0 | n/a | high |
| 19 | X | Plp | + | − | + | − | 2 | r, 7, 13, 21 | 0 | − | medium |
| 20 | X | X | + | + | + | + | 4 | s, 13, 21 | 0 | + | high |
| 21 | X | Pvx | + | + | + | − | 3 | r, 7, 13, 19, 20 | 0 | + | high |
| 22 | X | Hel | + | − | + | − | 2 | r, 24 | 0 | + | high |
| 23 | X | O3 | + | + | + | + | 4 | r, 14, 32, 33 | 0 | + | high |
| 24 | Hel | X | + | + | − | − | 2 | r, 22 | 0 | + | high |
| 25 | Hel | O3 | + | − | − | − | 1 | 0 | + | medium | |
| 26 | Rdrp | Plp | + | − | − | − | 1 | 16, 27, 28 | 10 | − | medium |
| 27 | Rdrp | X | + | + | − | − | 2 | 16, 26, 28 | 10 | − | medium |
| 28 | Rdrp | Pvx | + | + | − | − | 2 | r, 16, 26, 27 | 10 | + | high |
| 29 | Rdrp | O3 | + | + | − | − | 2 | 10 | + | high | |
| 30 | Rdrp | Rdrp | − | + | + | − | 2 | s | 10 | − | medium |
| 31 | O3 | Plp | − | − | + | − | 1 | r, 9, 14, 33 | 3 | + | high |
| 32 | O3 | X | + | + | + | + | 4 | r, 14, 23, 33 | 3 | n/a | high |
| 33 | O3 | Pvx | + | − | + | + | 3 | r, 9, 14, 23, 31, 33 | 3 | + | high |
| 34 | O3 | O3 | + | + | + | + | 4 | s | 3 | + | high |
| 35 | O2 | X | + | − | − | − | 1 | 0 | + | medium |
NN, NC, CN, CC indicates the nature of fusion proteins by which an interaction was or not detected (+ or −, respectively). Redundant PPI in Y2H: Interactions that could be potentially reproduced by a) more than one vector pairing, b) reciprocal interaction (r, found in both bait-prey and prey-bait orientation) or c) overlapping constructs (indicated by their consecutive number). Possible only if the constructs P, V, X or Pvx are involved. (s): Self-interaction. The quality of interactions was classified from “high quality” to “basic quality”. “high quality”: Redundant interaction found in Y2H and LuMPIS. “medium quality”: Redundant interaction found in Y2H or LuMPIS. “basic quality”: No redundant interaction detected. Interactions No. 15, 17 and 30 were detected by combinations of C-terminal tagged proteins.