Figure 8. Inhibitory effect of MUPCDH on cell proliferation.

(a) Knockdown of the MUPCDH gene increased cell survival in human renal cortical epithelial (HRCE) cells. (b) Overexpression of the MUPCDH gene decreased cell growth of WT9-7 cells. (c) 5-bromo-2′-deoxyuridine (BrdU) incorporation assays were carried out using HRCE cells that had been transfected with MUPCDH short interfering RNA (siRNA) or control siRNA. (d) Proliferating cell nuclear antigen (PCNA) staining carried out using HEK293T cells that overexpressed the MUPCDH gene. The level of PCNA expression was also measured by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). β-Actin was used as an internal control in real-time qRT-PCR assays. (e) Cell proliferation rate was measured using a cell proliferation assay kit (WST-1) following treatment of WT9-7 cells with 5-aza-2′-deoxycytidine (5 μM). The experiment was performed in triplicate. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.