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. 2015 Oct 14;5:15096. doi: 10.1038/srep15096

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(A) Schematic of the plasmid pCas9cr4 which has cas9 expressed under control of the P_TET promoter and tetR constitutively expressed. (B) Schematic of the plasmid pKDsg-xxx which has the sgRNA expressed under control of the P_TET promoter and the three genes that compose the λ-Red system under control of the arabinose inducible promoter P_araB.