Table 2. Efficiency of genome editing using the no-SCAR system.
| Plasmid | Mutation DNA | CFU’s1 | Positives2 | Control DNA | CFU’s1 | Positives2 | Fold-Change in CFU’s3 |
|---|---|---|---|---|---|---|---|
| pKDsg-ack | ack mut2 | 3.9 × 105 | 99 ± 1% | rpoB mut | 6.6 × 103 | 8 ± 1% | 61 ± 23 |
| pKDsg-ack | Ack CD | 5.5 × 104 | 85 ± 1% | ack mut2 | 6.6 × 103 | 8 ± 1% | 9.3 ± 1.9 |
| pKDsg-rpoB | rpoB | 1.7 × 105 | 94 ± 4% | Ackmut2 | 1.1 × 104 | 3 ± 1% | 16.6 ± 1.8 |
| pKDsg-pfkAE | dsDNA-ssrA | 8.0 × 105 | 100% | dsDNA-off | 2.5 × 105 | nd | 4.5 ± 1.8 |
1The CFU’s are the average of three independent experiments.
2The % of positive were obtained from chloroacetate and rifampicin selection plates.
3The Fold-Change in CFU’s is the average of the same three independent experiments ± SD.