Figure 1. Expression of N-WASP is lower than that of WASP in hematopoietic cells.

(A) qrtPCR analysis for WASP and N-WASP expression in Jurkat, THP-1 and Raji cells. HEK293T cells were used as negative control for analysis of WASP expression. ***P < 0.001 (B) Knockdown of endogenous WASP expression in Jurkat T-cells using WASP specific S1-WASP shRNA. (C) Expression of N-WASP in wild type and Jurkat^WKD T-cells. (D) mRNA level of N-WASP quantified by qrtPCR in wild type and Jurkat^WKD T-cells. (E) Exogenous expression of WASP and N-WASP in Jurkat^WKD T-cells.