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. 2015 Oct 14;5:15031. doi: 10.1038/srep15031

Figure 2. N-WASP expression does not rescue the WASP deficiency in Jurkat T-cells chemotaxis.

Figure 2

(A) Vector plots representing migration path of 20 randomly selected JurkatWKD T-cells expressing (1) RFP, (2) WASPR-RFP, (3) N-WASP-RFP in Dunn chamber assay exposed to a gradient of chemokine SDF-1α (maximum at top). The intersection point of X and Y axis was taken as starting point of each cell. (B) Overall directionality of migration (final position of cell in each 20° sector). (C) Migration velocity of total 60 randomly selected cells of cell type as in panel A. **P < 0.01 compared to RFP expressing JurkatWKD T-cells. (D) Transwell migration of JurkatWKD T-cells expressing (1) RFP, (2) WASPR-RFP, (3) N-WASP-RFP represent as percentage of cells migrated. **P < 0.01 compared to RFP expressing JurkatWKD T-cells.