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. 2015 Oct 14;5:15031. doi: 10.1038/srep15031

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Copyright © 2015, Macmillan Publishers Limited

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(A) WASP-I30 region was inserted in N-WASP between WH1 domain and basic region of N-WASP. (B) Jurkat T-cells expressing EGFP tagged WASP_R, WASP_R^ΔI30, N-WASP and N-WASP-I30 were stimulated on anti-CD3 (OKT3)-coated coverslip for 5 min and stained with Alexa fluor 594 phalloidin. The experiment has been repeated three times and 25 cells were analyzed each time. (C) Jurkat T-cells expressing WASP_R-RFP, WASP_R^ΔI30-RFP, N-WASP-RFP and N-WASP-I30-RFP were allowed to form conjugate with Raji B cell initially pulsed with SEE toxin. The conjugates between T: B cells were detected by Alexa fluor 488 phalloidin. Bar = 5 μm.