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. 2015 Jul 14;407(27):8321–8331. doi: 10.1007/s00216-015-8875-z

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Effect of EGF (50 ng/ml for 15 min) and erlotinib (10 μg/ml for 2 h) on ERK and AKT activation in SW-48, HT-29, and SW-480 colon cancer cells. Cell lysates were resolved by sodium dodecyl sulfate–Polyacrylamide gel electrophoresis and Western blot analysis with use of antibodies that recognise phosphorylated ERK1 and ERK2 (p-Erk 1/2) and phosphorylated AKT (p-Akt) or total ERK1 and ERK2 (Erk 1/2), and AKT (Akt)