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. 2015 Oct 14;5:15133. doi: 10.1038/srep15133

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(A,B) CLSM observation of ROS generation resulting from HA-mediated phototoxins in C. albicans cells cultured in YPD (A) or YPDO (B) medium. HA-treated C. albicans cells were stained with DCFHDA, which was rapidly oxidised by ROS into its fluorescent derivative DCF. The green fluorescence intensity of DCF was used to indicate the amount of generated ROS. (C,D) ROS production for HA-mediated photodynamic inactivation in C. albicans cultured in YPD (C) or YPDO (D) medium, revealed by flow cytometry detection. Black line, control; red line, 0.25 μg/ml of HA; green line, 0.5 μg/ml of HA; blue line, 1 μg/ml of HA; and purple line, 2 μg/ml of HA.