Table 1.
Plasmids
| Plasmid | Description | Source |
|---|---|---|
| pLHN12-His | pT7-His6-rpoD | (Panaghie et al., 2000) |
| pET15b-His-fliA | pT7-His6-fliA | This work |
| pFW11tet-PR′_+19 | λPR′ promoter and native σ70-dependent pause element with a second σ70-dependent pause element located 19 bp downstream of the +1 transcription start site. The tR′ intrinsic terminator is positioned to terminate transcription ∼116 bp downstream of +1. | (Deighan et al., 2011) |
| pFW11tet-mutPR′_+19 | Same as pFW11tet-PR′_+19 but with A+2 G/T+6 G mutations in the native σ70-dependent pause element. | (Deighan et al., 2011) |
| pFW11tet-Ptar_+19 (pNUN175) | Same as pFW11tet-PR′_+19 except that the promoter (up to and including +1) has been replaced with the σ28-dependent Ptar promoter. | This work |
| pFW11tet-Ptar_mut+19 (pNUN176) | Same as pFW11tet-Ptar_+19 but with mutations in the pause element. | This work |
| pBR-fliA | pSG585-fliA | This work |
| pSG585 | colE1 origin plasmid with lacUV5 upstream of multiple cloning site | This work |
| pNUN191 | pCDFlacMUT3-rpoD | This work |
| pCDFlacMUT3 | pCDFlac with attenuated −35 element (AATACA) | This work |
| pCDFlac | derivative of pCDF-1b into which the lacUV5 promoter has been inserted | (Montero-Diez et al., 2013) |