Fig. 6.

The expression of IL-1β by MRECs from TLR4 deficient mice under high glucose condition. MRECSs of wild type (WT) mice and TLR4 knockout (TLR4 KO) mice were cultured with 5.5 and 15 mmol/l glucose for 6 h (a) and 24 h (b). a The mRNA level of IL-1β was determined by real time PCR. Asterisk indicates P < 0.05 compared to WT mice. b IL-1β protein concentration in the supernatants of MRECs after high-glucose treatment for 24 h was measured using ELISA. Asterisk indicates P < 0.05 compared to WT mice