TMSCs maintain viability at least 4 months after anterior chamber injection. TUNEL assay was used to detect apoptotic cells on the cryosections of the eyes with cell injection. TUNEL assay stains apoptotic cells as red and DiO prelabeled cells before injection are green. (A, C, E) show the sections with TMSC-injection at 1 week, 4 weeks, and 4-months after injection. (B, D, F) show the sections with fibroblast injection at 1 week, 4 weeks, and 4 months after injection. The Schlemm's canal and the TM are pointed with arrows. DAPI stains nuclei as blue. Scale bars: 50 μm.