Fig. 8.

Upstream open reading frames (uORFs) are present in mouse and rat Gal₂ mRNA. Alignment of the 5′-UTR sequences of mouse and rat Gal₂ mRNA showing uORFs, with ATG triplets (bold underlined, red) and associated ORFs (bold, green) upstream of the Gal₂ coding region (‘Gal₂’; bold, blue) indicated, and note that of the six uORFs in the mouse sequence (uORF1-6) there is conservation of uORF3 and uORF6 in rat. For clarity, the open reading frame of mouse uORF4 is not indicated. Sequences shown are mouse (Mus) and rat mRNA reference sequences (respectively NM_010254 with 5′-UTR of 547 nt, and NM_019172 with 5′-UTR of only 20 nt), with additional rat 5′-UTR sequence derived from expressed sequence tag (EST) CB780446 and further extended by EST CB713787 to 466 nt. The rat sequence shares identity with the predicted rat Gal₂ mRNA sequence (XM_008768432) and rat hypothalamic cDNA clones including 5′-UTRs of 492 and 296 bp have been reported, though the sequences are not available (Howard et al., 1997; U94322). The mouse sequence is supported by cDNAs/ESTs including uORF1-6 (AK053776, BC116980, BB607282), uORF2-6 (BC116982) and uORF5-6 (AK132050, BY712835) isolated from embryonic day 12 embryo or head, neonatal day 0 eyeball, and brain.