Comparison of high (5 ×) to low (2.5 ×) magnification photomicrograph images of Gal1-mCherry immunofluorescence in brain. Lefthand column is high power magnification images (5 ×) from main article Fig. 7C–F and I, and Supplementary Fig. 6B, E and F (designations shown in brackets, top right of each image). Righthand column is corresponding low power magnification images (designated ‘2.5 ×’ in top left of each image). Additional brain areas specified here are the anterior hypothalamic area (AHP) and anterior part of basomedial amygdala (BMA) (Supplementary Fig. 7F). Note that 2.5 × magnification images of Supplementary Figs. 7H and L, respectively, also include the 5 × magnification images shown in Fig. 7G–H and Supplementary Fig. 6C–D. Scale bars are 0.5 mm (5 × objective) and 1 mm (2.5 × objective). Other abbreviations in alphabetical order are: aca, anterior part of anterior commissure; AHiPM, posteromedial hippocampal amygdala; aq, aqueduct; BMP, posterior part of basomedial amygdala nucleus; CB, cerebellum; CL, central lateral thalamic nucleus; CM, central medial thalamic nucleus; CPu, caudate putamen; DG, dentate gyrus; DM, dorsomedial ventromedial hypothalamic nucleus; DMPAG, dorsomedial part of periaqueductal grey; DRV, ventral part of dorsal raphe nucleus; IMD, intermediodorsal thalamic nucleus; LC, locus coeruleus; LPMC, lateral posterior thalamic nucleus; LSD, dorsal part of lateral septal nucleus; LSI, intermediate part of lateral septal nucleus; LSV, ventral part of lateral septal nucleus; MDM, medial part of mediodorsal thalamic nucleus; MHb, medial habenula; MM, medial mammillary nucleus; MnR, median raphe nucleus; MS, medial septal nucleus; PC, paracentral thalamic nucleus; PDTg, posterodorsal tegmental nucleus; PtN, pretectal nucleus; PV, paraventricular thalamic nucleus; S, subiculum; VLPAG, ventrolateral part of periaqueductal grey; VMH, ventromedial hypothalamic nucleus; 3V, 3rd ventricle; 4V, 4th ventricle.